Sister chromatid exchange (SCE) induced in vivo by 'pan masala', a betel quid ingredient, was studied in male mice. The mice were injected ip with an aqueous suspension of pan masala at doses of 5, 12.5, 25, 50, 100 or 200 mg pan masala/kg body weight. A significant dose-related increase in SCE was observed (Cochran-Armitage trend test). The minimum effective dose was 25 mg/kg. The two highest doses caused significant delays in the cell cycle. These results indicate that pan masala is a potential DNA-damaging agent and cytotoxic to bone marrow cells.