Functional conversion from HIV-1 Rev to HTLV-1 Rex by mutation

Biochem Biophys Res Commun. 1991 Aug 15;178(3):1226-32. doi: 10.1016/0006-291x(91)91024-7.

Abstract

A nucleolar localizing rev gene mutant M10 of human immunodeficiency virus type 1 (HIV-1) lost a Rev function completely, instead, gained a Rex activity of human T cell leukemia virus type 1 (HTLV-1). The obtained compatibility between Rev M10 and Rex with their own nucleolar targeting signal (NOS) suggests a common molecular mechanism of their post-transcriptional regulation, despite no sequence similarities of both proteins and their responsive RNA elements, respectively.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cell Nucleolus / physiology
  • Cell Nucleolus / ultrastructure
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Cloning, Molecular
  • Gene Conversion*
  • Gene Products, rev / metabolism
  • Gene Products, rex / metabolism
  • Genes, pX*
  • Genes, rev*
  • HIV-1 / genetics*
  • Human T-lymphotropic virus 1 / genetics*
  • Molecular Sequence Data
  • Mutation*
  • Plasmids
  • Transfection
  • rev Gene Products, Human Immunodeficiency Virus

Substances

  • Gene Products, rev
  • Gene Products, rex
  • rev Gene Products, Human Immunodeficiency Virus
  • Chloramphenicol O-Acetyltransferase