The glucose solution was broken down by focusing the 1.064 microm beam of a Nd : YAG laser, and the plasma was produced. The spectral signals were detected by an experimental setup including spectrograph and ICCD. The spectral line at 247.86 nm was identified as the characteristic of glucose by contrasting the spectra of glucose solution and pure water. Comparing the spectral intensities of three kinds of glucose solution with different concentrations (3%, 6% and 9%), the experimental result showed that the bigger the concentration, the stronger the spectral intensity,and the characteristic spectral intensities with the three concentrations present the trend of logarithm increase. At the same concentration, the time evolution curve of the characteristic spectra was obtained by changing the delay time of ICCD. It is concluded that the intensity of the characteristic spectra first increases and then decreases with the delay time. With the glucose solution concentration altering, the decay time of the characteristic spectra is nearly fixed, meaning that the decay time is independent of the concentration. The decay time of the characteristic spectra is about 300 ns. Furthermore, it was found that the characteristic spectral intensities of glucose solution with different concentrations reach the maximum at the same delay time.