Two-year study evaluating the potential loss of methicillin resistance in a methicillin-resistant Staphylococcus aureus culture collection

J Clin Microbiol. 2008 Oct;46(10):3494-7. doi: 10.1128/JCM.00654-08. Epub 2008 Aug 20.

Abstract

A reported loss of mecA prompted us to monitor 360 cryostocked methicillin-resistant Staphylococcus aureus strains for stability. Concurrently, 14 well-characterized strains were stored in a Microbank preservation system and subjected to multiple freeze-thaw events. There were no significant declines in the methicillin-resistant populations with either method over a two-year period.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • Colony Count, Microbial
  • Freezing
  • Gene Deletion
  • Methicillin Resistance*
  • Penicillin-Binding Proteins
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / genetics*

Substances

  • Bacterial Proteins
  • Penicillin-Binding Proteins
  • mecA protein, Staphylococcus aureus