Cryptic peptide derived from the rat neuropeptide FF precursor affects G-proteins linked to opioid receptors in the rat brain

Peptides. 2008 Nov;29(11):1988-93. doi: 10.1016/j.peptides.2008.07.012. Epub 2008 Jul 24.

Abstract

Recently, we reported the discovery of a novel amino acid sequence derived from the NPFF precursor NAWGPWSKEQLSPQA, which blocked the expression of conditioned place preference induced by morphine and reversed the antinociceptive activity of morphine (5mg/kg, s.c.) in the tail-immersion test in rats. Here, we name it as NPNA (Neuropeptide NA from its flanking amino acid residues). The synthetic peptide influenced the expression of mRNA coding for Galpha(i1), (i2), and (i3) subunits. The results provide further evidence that yet another bioactive sequence might be present within the NPFF precursor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / metabolism
  • Corpus Striatum / drug effects
  • Corpus Striatum / metabolism
  • GTP-Binding Protein alpha Subunit, Gi2 / biosynthesis
  • GTP-Binding Protein alpha Subunits / biosynthesis*
  • GTP-Binding Protein alpha Subunits, Gi-Go / biosynthesis
  • Hippocampus / drug effects
  • Hippocampus / metabolism
  • Male
  • Morphine / antagonists & inhibitors
  • Neuropeptides / pharmacology*
  • Pain Measurement
  • Peptide Fragments / pharmacology*
  • Protein Precursors / pharmacology*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar

Substances

  • GTP-Binding Protein alpha Subunits
  • Neuropeptides
  • Peptide Fragments
  • Protein Precursors
  • RNA, Messenger
  • neuropeptide FF precursor (85-99), rat
  • Morphine
  • GTP-Binding Protein alpha Subunit, Gi2
  • GTP-Binding Protein alpha Subunits, Gi-Go