Abstract
Genes of human neurotrophins NGF, BDNF, NT-3 were cloned, and the corresponding proteins and their fragments were expressed in Escherichia coli BL-21 (DE3lambda) cells. Their intracellular localization was determined. The conditions for isolation and purification of the target recombinant proteins and for folding of BDNF and NT-3 precursors were selected. The recombinant proprecursors of human neurotrophines have been shown to possess complex oligomeric structure.
MeSH terms
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Biopolymers
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Brain-Derived Neurotrophic Factor / biosynthesis
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Brain-Derived Neurotrophic Factor / chemistry*
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Brain-Derived Neurotrophic Factor / genetics
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Cloning, Molecular
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Humans
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Nerve Growth Factor / biosynthesis
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Nerve Growth Factor / chemistry*
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Nerve Growth Factor / genetics
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Neurotrophin 3 / biosynthesis
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Neurotrophin 3 / chemistry*
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Neurotrophin 3 / genetics
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Peptide Fragments / biosynthesis
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Peptide Fragments / chemistry
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Peptide Fragments / genetics
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Protein Denaturation
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Protein Folding
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Protein Precursors / biosynthesis
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Protein Precursors / chemistry
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Protein Precursors / genetics
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
Substances
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Biopolymers
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Brain-Derived Neurotrophic Factor
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Neurotrophin 3
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Peptide Fragments
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Protein Precursors
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Recombinant Proteins
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Nerve Growth Factor