Healthcare personnel is being highly exposed to the professional risk of Hephatitis B virus infection by accidental professional injuries, where direct contact with patients blood occurs. Number of researches indicate significance and role of accidental injuries, especially over injection needles, which is leading cause for professional HBV infections occurence. HBsAg has capability to provoke imune system if applied to a man, to HBs anti-bodies production achieving thus protection from Hephatitis B Virus. Recombinant vaccine against viruse Hephatitis B achieves high imunology protection. It is considered that appereance of anti-HBs titre >10 mlU/ml upon received three doses of vaccine provides reliable protection from HBV infection. Aim of the work is to prove rates of asymptomatic HBV infection amongst healthcare personnel and other employees in Sarajevo Healthcare Institution, and to research occurence and rate of anti-HBs titre at vaccinated Healthcare personnel in Sarajevo Healthcare Institution one month upon received third dose of vaccine. Out of 980 examinees 4, or 0.41%, had acute HBV infection within research period, 10 or 1.02% examinees had chronical accidental HBV infection, 18 or 1.83 % out of 980 examinees had previous asymptomatic HBV infection, as the consequence of accidental injury. Results of these researches indicated that 82.76 % or 96 healthcare workers has had reliable protection from HB virus infection one month upon third vaccine dose against HBV, since achieved titres where higher than 10 mlU/ml.