Objective: To breed good strains by protoplast fusion in Phellinus baumii.
Methods: 5.8S rDNA-ITS sequences, mycelial growth rate, mycelial biomass and exopolysaccharides (EPS) production of 7 strains in different origins were analyzed, respectively. Then the inactivated parent protoplast and the PEG induction were used in the process of protoplast fusion.
Results: The strain P. baumii SA02 and P. baumii SA04 were selected as the parent strains. R002, R003 and R005 had antagonistic effects with parent strains.
Conclusion: R002, R003 and R005 are fusants of the parent strains.