Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography

FEBS Lett. 2008 Aug 20;582(19):2950-6. doi: 10.1016/j.febslet.2008.07.022. Epub 2008 Jul 25.

Abstract

P-glycoprotein (ABCB1) is an ATP-binding cassette protein that is associated with the acquisition of multi-drug resistance in cancer and the failure of chemotherapy in humans. Structural insights into this protein are described using a combination of small angle X-ray scattering data and cryo-electron crystallography data. We have compared the structures with bacterial homologues, and discuss the development of homology models for P-glycoprotein based on the bacterial Sav1866 structure.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / chemistry*
  • Bacterial Proteins / chemistry
  • Cryoelectron Microscopy
  • Crystallography
  • Electrons
  • Humans
  • Protein Conformation
  • Scattering, Small Angle
  • X-Ray Diffraction

Substances

  • ABCB1 protein, human
  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Bacterial Proteins