Histology in reflux disease is still regarded as having low sensitivity and specificity. During the last three decades several histological parameters have been developed, including thickness of basal cell layer, length of epithelial papillae, intraepithelial inflammatory cells and dilation of intercellular spaces. Unfortunately classification of these parameters was often not related to the clinical symptoms of the reflux affected individuals, proper control subjects were often missing and often no interobserver variation was given. Another reason for the proposed low value of biopsies is the fact that biopsies were often taken in a non-standardised way. Recent studies point towards an area of predominantly epithelial damage close to the lesser curvature or around the right oesophageal wall. In parallel, some large, careful studies carried out recently showed a good correlation with the clinical picture of reflux disease due to a standardised biopsy protocol and proper controls. But biopsies are not recommended in all routine classical cases of reflux disease since it is believed that little further information in addition to endoscopy can be obtained. However, histology can deliver much more information than the diagnosis of reflux induced lesions (e.g. the exclusion of malignancy or other oesophageal diseases).