Ingress of blood-borne macrophages across the blood-brain barrier in murine HIV-1 encephalitis

J Neuroimmunol. 2008 Aug 30;200(1-2):41-52. doi: 10.1016/j.jneuroim.2008.06.009. Epub 2008 Jul 24.

Abstract

Blood-borne macrophage ingress into brain in HIV-1 associated neurocognitive disorders governs the tempo of disease. We used superparamagnetic iron-oxide particles loaded into murine bone marrow-derived macrophages (BMM) injected intravenously into HIV-1 encephalitis mice to quantitatively assess BMM entry into diseased brain regions. Magnetic resonance imaging tests were validated by histological coregistration and enhanced image processing. The demonstration of robust BMM migration into areas of focal encephalitis provide 'proof of concept' for the use of MRI to monitor macrophage ingress into brain.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood-Brain Barrier / pathology
  • Blood-Brain Barrier / physiopathology*
  • Cell Movement / physiology
  • Disease Models, Animal
  • Encephalitis / etiology*
  • Encephalitis / pathology*
  • Encephalitis / virology
  • Glial Fibrillary Acidic Protein / metabolism
  • HIV Infections / complications*
  • Imaging, Three-Dimensional
  • Macrophages / pathology
  • Macrophages / physiology*
  • Magnetic Resonance Imaging
  • Male
  • Mice
  • Mice, SCID
  • Vimentin / metabolism

Substances

  • Glial Fibrillary Acidic Protein
  • Vimentin