Abstract
The present study was designed to elucidate the role of TLR2, TLR4 and dectin-1 in the production of IL-12p40 by bone marrow-derived dendritic cells (BM-DCs) infected with Penicillium marneffei. IL-12p40 production was almost completely abrogated in BM-DCs from TLR2 gene-knockout (KO) and MyD88KO mice, but not from TLR4-defective C3H/HeJ mice compared to those from control mice. Furthermore, BM-DCs from dectin-1KO mice faintly produced IL-12p40 upon stimulation with this fungus. Using a luciferase reporter assay, P. marneffei activated NF-kappaB in HEK293 cells transfected with the TLR2 gene, but not with the dectin-1 gene, and their co-transfection did not lead to further increase in this response. These results indicate that TLR2 and dectin-1 are essential in sensing P. marneffei for the activation of BM-DCs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Analysis of Variance
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Animals
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Bone Marrow Cells / immunology
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Bone Marrow Cells / metabolism
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Bone Marrow Cells / microbiology
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Dendritic Cells / microbiology*
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Interleukin-12 Subunit p40 / biosynthesis*
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Lectins, C-Type
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Luciferases
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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NF-kappa B / metabolism
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Penicillium / immunology*
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Penicillium / metabolism
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Penicillium / pathogenicity
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Toll-Like Receptor 2 / genetics
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Toll-Like Receptor 2 / metabolism*
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Toll-Like Receptor 4 / genetics
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Toll-Like Receptor 4 / metabolism
Substances
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Interleukin-12 Subunit p40
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Lectins, C-Type
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Membrane Proteins
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NF-kappa B
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Nerve Tissue Proteins
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Toll-Like Receptor 2
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Toll-Like Receptor 4
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dectin 1
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Luciferases