Genus-specific PCR for molecular identification of novel isolates of the genus Nesterenkonia

Wei Sheng Wu Xue Bao. 2008 May;48(5):644-50.

Abstract

Objective and method: For rapid identification of Nesterenkonia species, one set of genus-specific primers was designed and synthesized for polymerase chain reaction based on the 16S rRNA gene sequences.

Results and conclusion: The genus specificity of these primers was validated with reference strains as well as with wild-type isolates. Partial sequencing results of 16S rRNA gene of the wild-type isolates confirmed that they are members of the genus Nesterenkonia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodiversity
  • DNA Primers / genetics
  • Micrococcaceae / classification
  • Micrococcaceae / genetics*
  • Micrococcaceae / isolation & purification*
  • Phylogeny
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S / genetics
  • Species Specificity

Substances

  • DNA Primers
  • RNA, Ribosomal, 16S