Abstract
Vaccines using bone marrow-derived dendritic cells (DCs) sensitized to Abeta 1-42 peptide and other mutant peptides were tested on BALB/c and APP(SW) transgenic mice. Wild type Abeta 1-42-sensitized DC vaccine (DCSV) produced no response, but all peptides with a T-cell epitope mutation induced antibody responses without inflammation. DCSV with Abeta 1-25 peptide with mutated T-cell epitope failed to induce antibody response, while DCSV with Abeta 1-35 with mutated T-cell epitope produced a strong antibody response. The entire T-cell epitope is required in a DC vaccine to induce antibody response. DCSV with Abeta peptide carrying the entire mutant T-cell epitope may be an appropriate vaccine against AD.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Alzheimer Vaccines / immunology*
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Amyloid beta-Peptides / genetics*
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Amyloid beta-Peptides / immunology*
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Amyloid beta-Peptides / pharmacology
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Amyloid beta-Protein Precursor / genetics
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Animals
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Antibody Formation / drug effects
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Antibody Formation / genetics
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Antibody Specificity / drug effects
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Antibody Specificity / genetics
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Bone Marrow / anatomy & histology
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Dendritic Cells / drug effects
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Dendritic Cells / immunology*
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Enzyme-Linked Immunosorbent Assay / methods
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Epitope Mapping
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Female
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Flow Cytometry / methods
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Humans
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Mice
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Mice, Inbred BALB C
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Mice, Transgenic
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Mutation*
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Peptide Fragments / genetics
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Peptide Fragments / pharmacology
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Time Factors
Substances
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Alzheimer Vaccines
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Amyloid beta-Peptides
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Amyloid beta-Protein Precursor
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Peptide Fragments
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amyloid beta-protein (1-42)