Separation-free detection of nuclear factor kappa B with double-stranded molecular probes

Analyst. 2008 Aug;133(8):998-1000. doi: 10.1039/b809113g. Epub 2008 Jun 24.

Abstract

This paper reports a simple, rapid molecular binding scheme for the detection and quantification of the transcription factor nuclear factor kappa B and other DNA binding proteins without any separation or immobilization step.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA
  • DNA-Binding Proteins / analysis
  • Fluorescent Dyes
  • Humans
  • Microscopy, Fluorescence
  • Molecular Probes
  • NF-kappa B / analysis*
  • Protein Binding

Substances

  • DNA-Binding Proteins
  • Fluorescent Dyes
  • Molecular Probes
  • NF-kappa B
  • DNA