Abstract
In the present work, a sensitive spectroscopic assay based on surface-enhanced Raman spectroscopy (SERS) using gold nanoparticles as substrates was developed for the rapid detection protein-protein interactions. Detection is achieved by specific binding biotin-modification antibodies with protein-stabilized 30 nm gold nanoparticles, followed by the attachment of avidin-modification Raman-active dyes. As a proof-of-principle experiment, a well-known biomolecular recognition system, IgG with protein A, was chosen to establish this new spectroscopic assay. Highly selective recognition of IgG down to 1 ng/ml in solution has been demonstrated.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / immunology
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Avidin / immunology
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Avidin / metabolism
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Binding Sites
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Biotin / immunology
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Biotin / metabolism
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Gold / chemistry*
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Immunoglobulin G / immunology
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Immunoglobulin G / metabolism*
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Metal Nanoparticles / chemistry*
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Nanotechnology / instrumentation
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Nanotechnology / methods*
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Particle Size
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Scattering, Radiation
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Sensitivity and Specificity
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Spectrum Analysis, Raman / instrumentation
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Spectrum Analysis, Raman / methods*
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Staphylococcal Protein A / immunology
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Staphylococcal Protein A / metabolism*
Substances
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Antibodies, Monoclonal
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Immunoglobulin G
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Staphylococcal Protein A
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Avidin
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Biotin
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Gold