Gold nanoparticle-based surface enhanced Raman scattering spectroscopic assay for the detection of protein-protein interactions

Anal Sci. 2008 Jul;24(7):907-10. doi: 10.2116/analsci.24.907.

Abstract

In the present work, a sensitive spectroscopic assay based on surface-enhanced Raman spectroscopy (SERS) using gold nanoparticles as substrates was developed for the rapid detection protein-protein interactions. Detection is achieved by specific binding biotin-modification antibodies with protein-stabilized 30 nm gold nanoparticles, followed by the attachment of avidin-modification Raman-active dyes. As a proof-of-principle experiment, a well-known biomolecular recognition system, IgG with protein A, was chosen to establish this new spectroscopic assay. Highly selective recognition of IgG down to 1 ng/ml in solution has been demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Avidin / immunology
  • Avidin / metabolism
  • Binding Sites
  • Biotin / immunology
  • Biotin / metabolism
  • Gold / chemistry*
  • Immunoglobulin G / immunology
  • Immunoglobulin G / metabolism*
  • Metal Nanoparticles / chemistry*
  • Nanotechnology / instrumentation
  • Nanotechnology / methods*
  • Particle Size
  • Scattering, Radiation
  • Sensitivity and Specificity
  • Spectrum Analysis, Raman / instrumentation
  • Spectrum Analysis, Raman / methods*
  • Staphylococcal Protein A / immunology
  • Staphylococcal Protein A / metabolism*

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin G
  • Staphylococcal Protein A
  • Avidin
  • Biotin
  • Gold