Fifteen fluoroquinolone-resistant strains of Escherichia coli were isolated from different clinical specimens of paediatric patients, 2 years after introduction of the drugs into Bangladesh. However, none of these patients had any definite history of treatment with fluoroquinolones. MICs of these strains to different antibiotics were determined by the broth microdilution method recommended by the Working Party of BSAC. Strains were highly resistant to all drugs except ceftriaxone. Study indicates a high frequency of fluoroquinolone-resistant E. coli strains among the clinical isolates which is possibly related to fluoroquinolone introduction.