After examining the definitions of persecutory and penitential types of guilt, based on Melanie Klein's view, we developed a single-item measure of these types and examined reliability and validity of the measure in three studies. Concurrent validity of the measure was shown among a university student population using the Test of Self-Conscious Affect-3 as an external validator. The questionnaire was not influenced by a socially desirable response style. Concurrent validity was demonstrated by studying different external variables: the two types of guilt feelings are moderately correlated with each other, but penitential guilt is more strongly correlated to variables related to ego maturation. Test-retest stability is only modest, suggesting that the constructs of measured guilt feelings may fluctuate over time.