Objective: To explore the appropriate amount of template DNA for Sinofiler Kit.
Methods: The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.
Results: It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.
Conclusion: Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.