[Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique]

Fa Yi Xue Za Zhi. 2008 Apr;24(2):129-30, 133.
[Article in Chinese]

Abstract

Objective: To explore the appropriate amount of template DNA for Sinofiler Kit.

Methods: The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.

Results: It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.

Conclusion: Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / analysis*
  • Forensic Medicine / methods*
  • Hair / chemistry
  • Humans
  • Microsatellite Repeats*
  • Reagent Kits, Diagnostic
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Templates, Genetic*

Substances

  • Reagent Kits, Diagnostic
  • DNA