Simultaneous determination of artemether and lumefantrine in fixed dose combination tablets by HPLC with UV detection

Isabela da Costa César; Fernando Henrique Andrade Nogueira; Gérson Antônio Pianetti

doi:10.1016/j.jpba.2008.05.022

Simultaneous determination of artemether and lumefantrine in fixed dose combination tablets by HPLC with UV detection

J Pharm Biomed Anal. 2008 Nov 4;48(3):951-4. doi: 10.1016/j.jpba.2008.05.022. Epub 2008 May 28.

Authors

Isabela da Costa César¹, Fernando Henrique Andrade Nogueira, Gérson Antônio Pianetti

Affiliation

¹ Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil. isaccesar@bol.com.br

PMID: 18602241
DOI: 10.1016/j.jpba.2008.05.022

Abstract

This paper describes the development and validation of a HPLC-UV method (210 nm) for the simultaneous quantitation of artemether and lumefantrine in fixed dose combination tablets. The method showed to be linear (r(2)>0.99), precise (R.S.D.<2.0%), accurate (recovery of 101.07% for artemether and 101.58% for lumefantrine), specific and robust. Four batches of artemether-lumefantrine tablets were assayed by the validated method. The artemether contents in the tablets varied from 98.61% to 103.35%, while lumefantrine contents were 97.92-100.48%.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antimalarials / analysis*
Antimalarials / chemistry
Artemether
Artemisinins / analysis*
Artemisinins / chemistry
Chromatography, High Pressure Liquid / methods*
Dosage Forms
Drug Combinations
Ethanolamines / analysis*
Ethanolamines / chemistry
Fluorenes / analysis*
Fluorenes / chemistry
Lumefantrine
Molecular Structure
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet / methods*
Tablets / analysis

Substances

Antimalarials
Artemisinins
Dosage Forms
Drug Combinations
Ethanolamines
Fluorenes
Tablets
Artemether
Lumefantrine