Stereoselective bioreduction of bulky-bulky ketones by a novel ADH from Ralstonia sp

Iván Lavandera; Alexander Kern; Bianca Ferreira-Silva; Anton Glieder; Stefaan de Wildeman; Wolfgang Kroutil

doi:10.1021/jo800849d

Stereoselective bioreduction of bulky-bulky ketones by a novel ADH from Ralstonia sp

J Org Chem. 2008 Aug 1;73(15):6003-5. doi: 10.1021/jo800849d. Epub 2008 Jul 3.

Authors

Iván Lavandera¹, Alexander Kern, Bianca Ferreira-Silva, Anton Glieder, Stefaan de Wildeman, Wolfgang Kroutil

Affiliation

¹ Research Centre Applied Biocatalysis c/o Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria.

PMID: 18597534
DOI: 10.1021/jo800849d

Abstract

Ketones with two bulky substituents, named bulky-bulky ketones, as well as less sterically demanding ketones were successfully reduced to the corresponding optically highly enriched alcohols using a novel identified recombinant short-chain alcohol dehydrogenase RasADH from Ralstonia sp. DSM 6428 overexpressed in E. coli.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Dehydrogenase / metabolism*
Catalysis
Escherichia coli / enzymology
Ketones / chemistry*
Ketones / metabolism*
Molecular Structure
Oxidation-Reduction
Ralstonia / enzymology*
Substrate Specificity
ras Proteins / metabolism

Substances

Ketones
Alcohol Dehydrogenase
ras Proteins