Abstract
Reporter clones of Staphylococcus aureus with different SOS response- and DNA repair-associated promoter-lux gene fusion constructs were constructed to study the effects of sub-MICs of antibiotics on the transcription of the SOS and methyl mismatch repair (MMR) genes. Fluoroquinolones (FQs) upmodulated both the SOS and the MMR genes. The patterns of antibiotic-induced transcriptional modulation were altered in FQ-resistant mutants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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DNA Mismatch Repair
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DNA Repair / drug effects*
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Drug Resistance, Bacterial
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Fluoroquinolones / pharmacology*
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Gene Expression Regulation, Bacterial*
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Humans
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Microbial Sensitivity Tests
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Promoter Regions, Genetic / genetics
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Promoter Regions, Genetic / physiology
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Recombinant Fusion Proteins / genetics
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SOS Response, Genetics / drug effects*
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / genetics
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Staphylococcus aureus / growth & development
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Staphylococcus aureus / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Anti-Bacterial Agents
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Arabidopsis Proteins
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Bacterial Proteins
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Fluoroquinolones
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Lux Arrhythmo protein, Arabidopsis
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Recombinant Fusion Proteins
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Transcription Factors