[RT-PCR-based methods for identification and typing of infectious hemopoietic necrosis virus in salmons]

Vopr Virusol. 2008 May-Jun;53(3):39-43.
[Article in Russian]

Abstract

A RT-PCR method has been developed to diagnose infectious hemopoietic necrosis virus (IHNV) in salmons. The authors show it possible to use the method for viral shedding in both a cell culture and a clinical sample from infected fishes. Genotyping of IHNV strains originating from North America, Europe, and Russia, by using the restriction fragment length polymerase analysis, has revealed that 10 of them belong to 3 existing genogroups (U, M, and L). Three Russian isolates are assigned into a separate subgroup. Phylogenetic analysis of several isolates has confirmed that viral strains from Katchatka belong to the North American U-genogroup whereas 3 Russian isolates from the continental zone of the country make up a separate subgroup within the same genogroup.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • DNA Primers
  • Fish Diseases / diagnosis*
  • Fish Diseases / virology
  • Genes, Viral
  • Infectious hematopoietic necrosis virus / classification*
  • Infectious hematopoietic necrosis virus / genetics
  • Infectious hematopoietic necrosis virus / isolation & purification*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Rhabdoviridae Infections / diagnosis
  • Rhabdoviridae Infections / veterinary*
  • Salmon / virology*
  • Sensitivity and Specificity

Substances

  • DNA Primers