Abstract
There is increasing evidence that protein kinase CK2 is involved, among a wide variety of cellular processes, in the maintenance of mammalian cell morphology and cell polarity. Here, we show that in epithelial cells, a fraction of CK2 is associated to the plasma membrane and that this localization is controlled by cell-matrix interactions. In addition, inhibition of CK2 activity in mammary epithelial cells (MCF10A), using either the specific CK2 inhibitor TBB or siRNA-mediated CK2beta knockdown, induced differential phenotypes revealing an important role of this enzyme in epithelial cell morphology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Casein Kinase II / antagonists & inhibitors
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Casein Kinase II / metabolism*
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Cell Line
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Cell Membrane / drug effects
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Cell Membrane / enzymology
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Cell Polarity* / drug effects
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Dogs
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Epithelial Cells / cytology*
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Epithelial Cells / drug effects
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Epithelial Cells / enzymology*
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Humans
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Mice
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Phenotype
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Protein Kinase Inhibitors / pharmacology
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Protein Subunits / metabolism
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Protein Transport / drug effects
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Subcellular Fractions / drug effects
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Subcellular Fractions / enzymology
Substances
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Protein Kinase Inhibitors
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Protein Subunits
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Casein Kinase II