Abstract
In response to the need for methods that can rapidly detect potentially virulent Bacillus anthracis spores, we developed a two-color flow cytometric assay capable of simultaneously identifying B. anthracis spores and the presence of spore-associated protective antigen, a virulence marker for strains harboring the pXO1 plasmid.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antigens, Bacterial / analysis*
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Bacillus anthracis / isolation & purification*
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Bacterial Toxins / analysis*
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Flow Cytometry / methods*
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Microscopy, Electron, Transmission
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Microscopy, Fluorescence
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Plasmids
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Spores, Bacterial / isolation & purification
Substances
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Antigens, Bacterial
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Bacterial Toxins
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anthrax toxin