Objective: To observe the changes of blood pressure (BP), pulse rate (PR) and the microstructure of celiac ganglion (CG) in rabbits with damaged CGs induced by alcohol and high intensity focused ultrasound.
Methods: Fourteen rabbits were randomly divided into two groups. The CGs of the rabbits in group A and group B were damaged by alcohol and high intensity focused ultrasound respectively. The changes of BP and PR 0, 1, 3, 5, and 10 minutes after the damage were recorded and compared. The microstructure changes of the damaged CGs were examined under optics microscope and electron microscope.
Results: Ganglionic morphology changes were obvious in both groups, with moved and concentrated karyons. In the CGs damaged by alcohol, the nucleolus still existed; some organelles could be identified; the myelination nerve fibre lost its myelin sheath or delaminated while the unmyelination nerve fibre exhibited vacuole formation. In the CGs damaged by high intensity focused ultrasound, all nucleolus disappeared, vacuole formed, intracellular membrane disappeared, axone locally necrotized. The BPs of the rabbits started to decrease three minutes after the alcohol treatment (P < 0.01), one minute after the high intensity focused ultrasound (P < 0.01). Significant differences of BP decline were observed between the two groups one minute after the CG damages. (P < 0.01). The PRs of the rabbits increased 5 minutes and 10 minutes after the high intensity focused ultrasound (P < 0.05, P < 0.01).
Conclusion: Using high intensity focused ultrasound to damage CGs has more significant impacts on BPs and PRs than alcohol.