Previous researchers have used the attitudes toward lesbians and gay men (ATLG) scale (Herek, 1988) and reported their own reliability coefficients and validity measures, but research on this subject is scarce in Chile. In order to determine if ATLG scale was a reliable and valid instrument in our country, we adapted and examined the psychometrics properties using a sample composed of 142 psychology and economics undergraduate students. This study found the ATLG scale to be reliable (a = .90) and valid for the Chilean population and is a recommended instrument for measuring attitudes toward gay men and lesbians.