Toxoplasmosis caused by Toxoplasma gondii is a protozoan infection found worldwide. It usually produces non-specific symptoms, but in pregnant women and immunocompomised individuals, it may cause severe and fatal illness. Many serological studies have been done in various parts of the world, but information is lacking for Vietnam. A seroprevalence study of T gondii antibodies in Vietnamese villagers (n = 650) was performed using the Sabin-Feldman dye test. The average seroprevalence was 4.19% (95% CI = 1.78-4.62), including 6.36% (95% CI = 3.22-11.09), 4.73% (95% CI = 1.92-9.50) and 1.09% (95% CI = 0.23-3.15) from Nghe An, Lao Cai and Tien Giang provinces, respectively. This study confirmed the low prevalence of toxoplasmosis in Vietnam similar to other countries in the region. Further studies are necessary in order to provide a complete picture for the country.