Selective enrichment of cysteine-containing peptides using SPDP-functionalized superparamagnetic Fe(3)O(4)@SiO(2) nanoparticles: application to comprehensive proteomic profiling

J Proteome Res. 2008 Aug;7(8):3591-6. doi: 10.1021/pr800067x. Epub 2008 Jun 19.

Abstract

Superparamagnetic Fe(3)O(4)@SiO(2) core-shell nanoparticles (ca. 30 nm diameter), which are surface-modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Liquid
  • Cysteine / analysis*
  • Ferrosoferric Oxide*
  • Indicators and Reagents
  • Nanoparticles*
  • Peptides / analysis*
  • Peptides / isolation & purification
  • Proteomics / methods*
  • Silicon Dioxide*
  • Succinimides*
  • Tandem Mass Spectrometry

Substances

  • Indicators and Reagents
  • Peptides
  • Succinimides
  • N-succinimidyl 3-(2-pyridyldithio)propionate
  • Silicon Dioxide
  • Cysteine
  • Ferrosoferric Oxide