Increased life span of humans and dynamic development of prosthetic treatment has caused that increased number of people are using removable dentures for longer periods of time. The materials used to make those prosthesis are becoming a potential pathogen factor for oral mucosa being in contact with this material. Prosthetic stomatopathy occurs in 20% to 70% of the patients who are using removable dentures. The present paper evaluates and compares adhesion of bacterial plague to the most common materials used for removable dentures i.e. acrylic material Vertex R.S., metal alloy used for frame prosthesis and a material which is an alternate to acrylic resin and acetal metal-resin Acetal Pressing D. Samples were made from the above mentioned materials and placed in mediums with four basic bacteria cultures and fungus Candida albicans (fungus) as the adhesion of bacterial plague to individual materials was evaluated. Such an evaluation facilitates choice of appropriate prosthetic material, allowing to make prosthetic restoration that is functional and aesthetic at the same time taking into account prosthetic stomatopathy prevention.