Tetrazolium salts for MIC determination in microplates: why? Which salt to select? How?

Marion Grare; Stéphane Fontanay; Coralie Cornil; Chantal Finance; Raphaël Emmanuel Duval

doi:10.1016/j.mimet.2008.05.010

Tetrazolium salts for MIC determination in microplates: why? Which salt to select? How?

J Microbiol Methods. 2008 Sep;75(1):156-9. doi: 10.1016/j.mimet.2008.05.010. Epub 2008 May 15.

Authors

Marion Grare¹, Stéphane Fontanay, Coralie Cornil, Chantal Finance, Raphaël Emmanuel Duval

Affiliation

¹ SRSMC, Nancy-University, CNRS, France.

PMID: 18556078
DOI: 10.1016/j.mimet.2008.05.010

Abstract

We reported evaluation of a colorimetric MIC assessment for routine susceptibility testing of non-fastidious bacteria, with addition of growth indicators (INT and MTT). Our results made us postulate that the use of such indicators was unnecessary for MIC determination in routine microdilution method.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacteria / drug effects*
Bacteria / growth & development*
Bacteria / metabolism
Colorimetry
Microbial Sensitivity Tests / methods*
Microbial Viability / drug effects
Tetrazolium Salts / metabolism*

Substances

Anti-Bacterial Agents
Tetrazolium Salts