Abstract
Standard polymerase chain reaction methods often cannot detect drug-resistance mutations in Plasmodium falciparum infections if the mutation is present in < or = 20% of the parasites. A heteroduplex tracking assay was developed that can detect dihydrofolate reductase 164-L mutations in variants representing 1% of the parasites in an individual host. Using this assay, we confirmed the presence of the mutation in P. falciparum infections in Malawi.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cohort Studies
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DNA Primers
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Female
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Humans
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Malaria, Falciparum / enzymology
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Malaria, Falciparum / genetics*
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Malawi
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Mutation*
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Nucleic Acid Heteroduplexes*
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Plasmodium falciparum / enzymology
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Polymerase Chain Reaction
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Pregnancy
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Pregnancy Complications, Parasitic / genetics
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Sensitivity and Specificity
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Tetrahydrofolate Dehydrogenase / genetics*
Substances
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DNA Primers
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Nucleic Acid Heteroduplexes
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Tetrahydrofolate Dehydrogenase