Abstract
Lens epithelium-derived growth factor (LEDGF) can be alternatively spliced to produce two isoforms-LEDGFp52 and LEDGFp75, however, LEDGFp52 has rarely been investigated. The LEDGFp52 protein or its monoclonal antibody was added to primary rat retinal ganglion cell cultures and their impact on neurite number and length, and the mRNA and protein expression levels of GAP-43, NF-L and MAP-2 quantified. LEDGFp52 was compared to the addition of ciliary neurotrophic factor (CNTF). LEDGFp52 protein significantly increased primary neurite growth compared to control conditions. In addition, the expression of GAP-43, NF-L and MAP2 genes and proteins were also significantly up-regulated. The positive action of the LEDGFp52 protein was similar to or more efficacious than CNTF. LEDGFp52 appears to be an important regulatory protein for the growth of cell processes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / pharmacology
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Ciliary Neurotrophic Factor / pharmacology*
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GAP-43 Protein / genetics
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GAP-43 Protein / metabolism
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Gene Expression / drug effects
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Intercellular Signaling Peptides and Proteins / immunology
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Intercellular Signaling Peptides and Proteins / pharmacology*
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism
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Neurites / drug effects*
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Neurites / physiology
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Neurofilament Proteins / genetics
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Neurofilament Proteins / metabolism
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Organ Culture Techniques
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RNA, Messenger / metabolism
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Rats
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Rats, Long-Evans
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Retina / cytology*
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Retinal Ganglion Cells / cytology*
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Retinal Ganglion Cells / drug effects
Substances
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Antibodies
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Ciliary Neurotrophic Factor
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GAP-43 Protein
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Intercellular Signaling Peptides and Proteins
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MAP2 protein, rat
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Microtubule-Associated Proteins
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Neurofilament Proteins
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RNA, Messenger
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lens epithelium-derived growth factor
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neurofilament protein L