Structure of a SARS coronavirus-derived peptide bound to the human major histocompatibility complex class I molecule HLA-B*1501

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jun 1;64(Pt 6):459-62. doi: 10.1107/S1744309108012396. Epub 2008 May 17.

Abstract

The human leukocyte antigen (HLA) class I system comprises a highly polymorphic set of molecules that specifically bind and present peptides to cytotoxic T cells. HLA-B*1501 is a prototypical member of the HLA-B62 supertype and only two peptide-HLA-B*1501 structures have been determined. Here, the crystal structure of HLA-B*1501 in complex with a SARS coronavirus-derived nonapeptide (VQQESSFVM) has been determined at high resolution (1.87 A). The peptide is deeply anchored in the B and F pockets, but with the Glu4 residue pointing away from the floor in the peptide-binding groove, making it available for interactions with a potential T-cell receptor.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites / immunology
  • Crystallography, X-Ray
  • HLA-B Antigens / chemistry*
  • HLA-B Antigens / genetics
  • HLA-B Antigens / immunology
  • Histocompatibility Antigens Class I / genetics
  • Histocompatibility Antigens Class I / isolation & purification
  • Histocompatibility Antigens Class I / metabolism*
  • Humans
  • Hydrogen Bonding
  • Hydrophobic and Hydrophilic Interactions
  • Ligands
  • Models, Molecular
  • Mutagenesis, Site-Directed
  • Oligopeptides / chemical synthesis
  • Oligopeptides / chemistry
  • Oligopeptides / immunology*
  • Oligopeptides / isolation & purification
  • Oligopeptides / metabolism*
  • Protein Binding / immunology
  • Protein Structure, Secondary
  • Severe acute respiratory syndrome-related coronavirus / chemistry*
  • Severe acute respiratory syndrome-related coronavirus / immunology
  • Water / chemistry

Substances

  • HLA-B Antigens
  • Histocompatibility Antigens Class I
  • Ligands
  • Oligopeptides
  • Water