This paper reports the results of an ethnobotanical survey of medicinal plants used by the Thari people of the Nara Desert, Sindh, Pakistan. Fifty-one plant species distributed across 28 families and 43 genera were discovered to have medicinal uses by local inhabitants of the Nara Desert. Twenty-one of those species are proposed to have new uses not recorded in the Indo-Pak folk herbal medicinal literature. Boraginaceae and Amaranthaceae were the most dominant families (5 species; 2 and 4 genera, respectively) of medicinal plants, followed by Asteraceae, Capparidaceae, Convolvulaceae, Poaceae, Scrophulariacea and Zygophyllaceae. About 44 types of ailments were treated with various parts of the 51 medicinal plant species. For treating ailments, the use of the whole plant was higher (53%) than leaves (18%), roots (14%) and fruits (10%) alone.