Abstract
We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Fluorescence
-
Green Fluorescent Proteins / chemistry*
-
Green Fluorescent Proteins / genetics
-
Green Fluorescent Proteins / metabolism*
-
HeLa Cells
-
Humans
-
Hydrogen-Ion Concentration
-
Microscopy, Fluorescence
-
Molecular Structure
-
Time Factors
Substances
-
Green Fluorescent Proteins