Identification of a receptor protein for neuropeptide Y in rabbit kidney. G-protein association and inhibition of adenylate cyclase

FEBS Lett. 1991 Feb 25;279(2):219-22. doi: 10.1016/0014-5793(91)80153-t.

Abstract

We previously identified the receptor for neuropeptide Y (NPY) on rabbit kidney membranes as a 100 kDa protein [(1990) J. Biol. Chem. 265, 18142-18143]. It is demonstrated in this study that the stable guanine nucleotide analogue, guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), is capable of decreasing the labeling of the 100 kDa protein in a concentration-dependent manner, with an IC50 value of 50 microM. This suggests that the 100 kDa protein represents a G-protein coupled receptor type. In isolated proximal tubules from rabbit kidney NPY decreases the parathyroid hormone stimulated cAMP production in a dose-dependent fashion. This indicates that the NPY receptor in rabbit kidney is negatively coupled to adenylate cyclase by a G1-like protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / metabolism*
  • Affinity Labels
  • Animals
  • Cross-Linking Reagents
  • Cyclic AMP / biosynthesis
  • GTP-Binding Proteins / metabolism*
  • Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
  • Kidney Tubules, Proximal / metabolism*
  • Neuropeptide Y / metabolism*
  • Rabbits
  • Receptors, Neuropeptide Y
  • Receptors, Neurotransmitter / metabolism*
  • Signal Transduction

Substances

  • Affinity Labels
  • Cross-Linking Reagents
  • Neuropeptide Y
  • Receptors, Neuropeptide Y
  • Receptors, Neurotransmitter
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Cyclic AMP
  • GTP-Binding Proteins
  • Adenylyl Cyclases