Aqueous suspension of carbon nanotubes enhances the specificity of long PCR

Biotechniques. 2008 Apr;44(4):537-8, 540, 542, passim. doi: 10.2144/000112692.

Abstract

DNA manipulation technology is facing more challenges in the postgenomics era. More and more nanomaterials have been investigated for their potential implications in developing better gene technology. In this study, we reported the beneficial effect of single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) in enhancing the specificity and total efficiency of long (14 kb) PCR. Hydroxylic and carboxylic carbon nanotubes (CNTs) had similar enhancing effects. Nanotubes could become another component for improvements in the amplification of long DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage lambda / genetics
  • DNA, Viral / genetics
  • DNA-Directed DNA Polymerase / chemistry
  • Nanotechnology / methods*
  • Nanotubes, Carbon / chemistry*
  • Polymerase Chain Reaction / methods*
  • Powders
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Templates, Genetic
  • Trehalose / chemistry
  • Viral Proteins
  • Water / chemistry

Substances

  • DNA, Viral
  • Nanotubes, Carbon
  • Powders
  • Viral Proteins
  • Water
  • gene 9 protein, Rotavirus B
  • Trehalose
  • DNA-Directed DNA Polymerase