Characterization of fluoroalcohols-induced intermediates of Mucor miehei lipase at low pH

Protein Pept Lett. 2008;15(4):346-52. doi: 10.2174/092986608784246425.

Abstract

We have previously characterized an acid-unfolded (U(A)) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with beta-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. alpha-Helical states accumulate at 80% (v/v) TFE and 30% (v/v) HFIP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Circular Dichroism
  • Hydrogen-Ion Concentration
  • Lipase / chemistry*
  • Mucor / enzymology*
  • Propanols / pharmacology*
  • Protein Conformation / drug effects
  • Protein Denaturation
  • Protein Folding
  • Protein Structure, Secondary / drug effects
  • Spectrometry, Fluorescence
  • Temperature
  • Trifluoroethanol / pharmacology*

Substances

  • Propanols
  • hexafluoroisopropanol
  • Trifluoroethanol
  • Lipase