Abstract
We have previously characterized an acid-unfolded (U(A)) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with beta-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. alpha-Helical states accumulate at 80% (v/v) TFE and 30% (v/v) HFIP.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Circular Dichroism
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Hydrogen-Ion Concentration
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Lipase / chemistry*
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Mucor / enzymology*
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Propanols / pharmacology*
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Protein Conformation / drug effects
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Protein Denaturation
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Protein Folding
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Protein Structure, Secondary / drug effects
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Spectrometry, Fluorescence
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Temperature
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Trifluoroethanol / pharmacology*
Substances
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Propanols
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hexafluoroisopropanol
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Trifluoroethanol
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Lipase