Abstract
The electrochemical analysis of the protein kinase, casein kinase, is accomplished by the voltammetric response of Ag(+) ions associated with the phosphorylated product; the sensing surface is regenerated by the cleavage of the phosphorylated product with alkaline phosphatase, and the phosphorylation/de-phosphorylation processes are monitored by XPS and contact angle measurements.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / chemistry
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Adenosine Triphosphate / metabolism
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Alkaline Phosphatase / chemistry*
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Alkaline Phosphatase / metabolism
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Binding Sites
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Casein Kinases / chemistry*
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Casein Kinases / metabolism
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Electrochemistry / methods
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Electrodes
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Oligopeptides / chemistry
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Oligopeptides / metabolism
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Phosphorylation
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Silver / chemistry
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Spectrum Analysis
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X-Rays
Substances
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Oligopeptides
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Silver
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Adenosine Triphosphate
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Casein Kinases
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Alkaline Phosphatase