Abstract
Novel groups of uncultivable anaerobic thermophiles were isolated from compost by enrichment cultivation in medium with a cell-free extract of Geobacillus toebii. The cell-free extract of G. toebii provided the medium with growth-supporting factors (GSF) needed to cultivate the previously uncultured microorganisms. Twenty-nine GSF-requiring candidates were successfully cultivated, and 16 isolated novel bacterial strains were classified into three different groups of uncultivable bacteria. The similarity among these 16 isolates and a phylogenetic analysis using 16S rRNA gene sequences revealed that these GSF-requiring strains represented novel groups within the family Clostridiaceae.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anaerobiosis
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Bacillaceae / classification
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Bacillaceae / genetics
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Bacillaceae / growth & development*
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Bacillaceae / isolation & purification*
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Cell Culture Techniques*
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Culture Media / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / genetics
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Molecular Sequence Data
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Phylogeny
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RNA, Ribosomal, 16S / genetics
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Sequence Homology
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Soil Microbiology*
Substances
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Culture Media
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S
Associated data
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GENBANK/EU178824
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GENBANK/EU178825
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GENBANK/EU178826
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GENBANK/EU178827
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GENBANK/EU178828
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GENBANK/EU178829
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GENBANK/EU178830
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GENBANK/EU178831
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GENBANK/EU178832
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GENBANK/EU178833
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GENBANK/EU178834
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GENBANK/EU178835
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GENBANK/EU178836
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GENBANK/EU178837
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GENBANK/EU178838
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GENBANK/EU178839