Abstract
Sub-additivity of antioxidant activities in assays of total antioxidant capacity has been reported previously and ascribed to binding of low-molecular weight antioxidants such as flavonoids by proteins. We demonstrate that this phenomenon is much more common and concerns also interactions between typical low-molecular weight oxidants in the assays of ABTS- decolorization and protection of fluorescein from AAPH-induced bleaching. The subadditive interactions between antioxidants may affect quantitative considerations drawn from in vitro assays of antioxidant capacity of biological samples.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / chemistry*
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Antioxidants / metabolism*
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Area Under Curve
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Benzothiazoles / analysis*
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Benzothiazoles / metabolism
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Drug Interactions
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Drug Synergism
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Flavonoids / chemistry*
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Fluorescein / analysis*
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Fluorescein / metabolism
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Free Radical Scavengers
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Indicators and Reagents
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Molecular Weight
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Oxidation-Reduction
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Sulfonic Acids / analysis*
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Sulfonic Acids / metabolism
Substances
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Antioxidants
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Benzothiazoles
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Flavonoids
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Free Radical Scavengers
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Indicators and Reagents
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Sulfonic Acids
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2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
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Fluorescein