Abstract
mAtNOS1 is a novel gene recently reported in mammalian genome with functions that are not fully understood. The present study shows that in human mammary adenocarcinoma MCF-7 cells, mAtNOS1 expression increases mitochondrial nitric oxide and calcium. Our study further shows that overexpression of mAtNOS1 induces apoptosis in MCF-7 cells by increasing mitochondrial protein tyrosine nitration and cytochrome c release. The present study suggests a novel function for mAtNOS1 in regulating mitochondrial nitric oxide and calcium and inducing apoptosis of MCF-7 cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / metabolism
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Adenocarcinoma / pathology*
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Apoptosis*
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Arabidopsis Proteins / biosynthesis*
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology*
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Calcium / metabolism
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Cell Line, Tumor
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Cytochromes c / metabolism
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Female
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Humans
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Immunochemistry
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Mitochondria / metabolism
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Nitric Oxide / metabolism
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Nitric Oxide Synthase / biosynthesis*
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Transfection
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Tyrosine / metabolism
Substances
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Arabidopsis Proteins
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Nitric Oxide
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Tyrosine
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Cytochromes c
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NOA1 protein, Arabidopsis
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Nitric Oxide Synthase
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Calcium