Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity

Mol Biosyst. 2008 Mar;4(3):246-8. doi: 10.1039/b717705d. Epub 2008 Jan 31.

Abstract

Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescens Pf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.

MeSH terms

  • Acyltransferases / chemistry
  • Acyltransferases / genetics*
  • Acyltransferases / metabolism*
  • Amino Acid Sequence
  • Catalysis
  • Databases, Protein
  • Directed Molecular Evolution*
  • Genetic Variation / genetics*
  • Kinetics
  • Molecular Sequence Data
  • Mutation / genetics
  • Protein Engineering*
  • Pseudomonas fluorescens / enzymology*
  • Pseudomonas fluorescens / genetics*

Substances

  • Acyltransferases
  • flavanone synthetase