Abstract
Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescens Pf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.
MeSH terms
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Acyltransferases / chemistry
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Acyltransferases / genetics*
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Acyltransferases / metabolism*
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Amino Acid Sequence
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Catalysis
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Databases, Protein
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Directed Molecular Evolution*
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Genetic Variation / genetics*
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Kinetics
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Molecular Sequence Data
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Mutation / genetics
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Protein Engineering*
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Pseudomonas fluorescens / enzymology*
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Pseudomonas fluorescens / genetics*
Substances
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Acyltransferases
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flavanone synthetase