Expression, purification, and characterization of aspartic endopeptidases: Plasmodium plasmepsins and "short" recombinant human pseudocathepsin

Abstract

The unit describes a basic protocols utilized to obtain milligram amounts of enzymatically active, pure recombinant Plasmodium plasmepsins and "short" human pseudocathepsin D. Specific details for the expression and purification of Plasmodium falciparum plasmepsin 2 and "short" human pseudocathepsin D in zymogen form are described in this chapter. The plasmepsin 2 protocols are also applicable to Plasmodium vivax, P. ovale, and P. malariae plasmepsins, as well as P. falciparum plasmepsin 4.