Latino youth in the United States are at higher risk for negative sexual outcomes compared to their European American counterparts. Adherence to traditional sexual values may protect against or increase their risk. Past studies have generally utilized proxy measures, such as acculturation, to assess sexual values. The objective of the current study was to develop and test culturally based sexual values measures among Latino youth. Focus groups and qualitative interviews were conducted to generate themes related to sexual values. Six measures were developed: three related to gender role norms (Sexual Talk as Disrespectful, Satisfaction of Sexual Needs as Important, Female Virginity as Important) and three related to comfort regarding sexuality (Comfort with Sexual Communication, Sexual Comfort, and Sexual Self-Acceptance). The scales' psychometric properties were assessed in a sample of 694 sexually active young Latinos. Results indicate that these measures conformed to single-factor scales and displayed acceptable reliabilities. Correlations with conceptually related measures were in hypothesized directions. Findings suggest it is feasible to directly assess sexual values in a valid and reliable manner. The measures presented in the current article represent tools for such assessment.