Evaluation of a new matrix-free laser desorption/ionization method through statistic studies: comparison of the DIAMS (desorption/ionization on self-assembled monolayer surface) method with the MALDI and TGFA-LDI techniques

Matthieu Bounichou; Lionel Sanguinet; Kamal Elouarzaki; Olivier Alévêque; Marylène Dias; Eric Levillain; David Rondeau

doi:10.1002/jms.1414

Evaluation of a new matrix-free laser desorption/ionization method through statistic studies: comparison of the DIAMS (desorption/ionization on self-assembled monolayer surface) method with the MALDI and TGFA-LDI techniques

J Mass Spectrom. 2008 Dec;43(12):1618-26. doi: 10.1002/jms.1414.

Authors

Matthieu Bounichou¹, Lionel Sanguinet, Kamal Elouarzaki, Olivier Alévêque, Marylène Dias, Eric Levillain, David Rondeau

Affiliation

¹ Université d'Angers, CIMA CNRS, UFR Sciences, 2 Bd Lavoisier, 49045 Angers, France.

PMID: 18416434
DOI: 10.1002/jms.1414

Abstract

This work demonstrates that the desorption/ionization on self-assembled monolayer surface (DIAMS) mass spectrometry, a recent matrix-free laser desorption/ionization (LDI) method based on an organic target plate, is as statistically repeatable and reproducible as matrix assisted laser desorption ionization (MALDI) and thin gold film-assisted laser desorption/ionization (TGFA-LDI) mass spectrometries. On lipophilic DIAMS of target plates with a mixture of glycerides, repeatability/reproducibility has been estimated at 15 and 30% and the relative detection limit has been evaluated at 0.3 and 3 pmol, with and without NaI respectively. Salicylic acid and its d(6)-isomer analysis confirm the applicability of the DIAMS method in the detection of compounds of low molecular weight.