Abstract
A fluorescent dye library approach for the development of a bioanalyte sensor was sought. The screening of a rosamine dye library against diverse macromolecules led to the discovery of a highly sensitive human serum albumin binder, G13, with approximately 36-fold fluorescence intensity change. G13 showed a highly selective response to HSA over other macromolecules including albumins from other species. The potential use of G13 for the detection of HSA in biofluids is described.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biosensing Techniques*
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Cattle
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Combinatorial Chemistry Techniques*
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Fluorescence
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Fluorescent Dyes / chemistry*
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Humans
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Molecular Structure
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Sensitivity and Specificity
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Serum Albumin / analysis*
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Serum Albumin / chemistry*
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Serum Albumin, Bovine / analysis
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Serum Albumin, Bovine / chemistry
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Small Molecule Libraries / chemistry*
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Species Specificity
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Stereoisomerism
Substances
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Fluorescent Dyes
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Serum Albumin
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Small Molecule Libraries
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Serum Albumin, Bovine