Abstract
Bioassay-guided chromatographic purification of the antitubercular chloroform extract of Pandanus tectorius Soland. var. laevis leaves afforded a new tirucallane-type triterpene, 24,24-dimethyl-5 beta-tirucall-9(11),25-dien-3-one (1), squalene and a mixture of the phytosterols stigmasterol and beta-sitosterol. Microplate Alamar Blue Assay (MABA) showed that 1 inhibited the growth of Mycobacterium tuberculosis H(37)Rv with a MIC of 64 microg/mL, while squalene and the sterol mixture have MICs of 100 and 128 microg/mL, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antitubercular Agents / chemistry
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Antitubercular Agents / isolation & purification
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Antitubercular Agents / pharmacology*
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Microbial Sensitivity Tests
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Mycobacterium tuberculosis / drug effects
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Mycobacterium tuberculosis / growth & development
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Pandanaceae / chemistry*
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Phytosterols / chemistry
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Phytosterols / isolation & purification
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Phytosterols / pharmacology*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plant Leaves / chemistry*
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Sitosterols / chemistry
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Sitosterols / isolation & purification
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Sitosterols / pharmacology
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Squalene / chemistry
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Squalene / isolation & purification
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Squalene / pharmacology
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Stigmasterol / chemistry
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Stigmasterol / isolation & purification
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Stigmasterol / pharmacology
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Triterpenes / chemistry
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Triterpenes / isolation & purification
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Triterpenes / pharmacology*
Substances
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24,24-dimethyltirucall-9(11),25-dien-3-one
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Antitubercular Agents
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Phytosterols
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Plant Extracts
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Sitosterols
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Triterpenes
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gamma-sitosterol
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Squalene
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Stigmasterol