Abstract
The rapid and accurate identification of Escherichia coli O157:H7 strains is central to reducing the impact of outbreaks. A real-time PCR-based approach to differentiating major outbreak lineages of O157 with novel single-nucleotide polymorphisms is described. The utility of this method is in detection of hypervirulent strains in cases of clinical disease.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
MeSH terms
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Bacterial Typing Techniques*
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DNA Primers
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DNA, Bacterial / analysis
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Escherichia coli Infections / microbiology
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Escherichia coli O157 / classification*
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Escherichia coli O157 / genetics*
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Escherichia coli O157 / isolation & purification
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Escherichia coli Proteins / genetics
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Humans
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Polymerase Chain Reaction / methods*
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Polymorphism, Single Nucleotide / genetics*
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Sensitivity and Specificity
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Time Factors
Substances
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DNA Primers
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DNA, Bacterial
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Escherichia coli Proteins